![]() One section, including any area suspicious for extracapsular extension, is sufficient. Grossly positive nodes do not need to be submitted in toto.Alternative approach: ink nodes in different colors to allow the halves to be matched up (“differential inking”).Bisected nodes should go in their own cassette(s) so that the two halves can be put back together.Describe any grossly identifiable disease. AE1/3 stain has been validated on previously frozen tissue. This is no longer standard, but it is not wrong. Note: The surgeons may occasionally request a frozen section on a sentinel lymph node. The specimens are radioactive, but the dose to the pathologist in one study was below the limit of detection (Klausen TL, Clin Physiol Funct Imaging 2005 Jul 25(4):196-202).These are handled identically and the designation has no significance to the pathologist. The nodes may be designated “hot” (meaning radiotracer was found in the node” and/or “blue” (meaning dye was found in the node).If surgeon does not rapidly identify node, the radiotracer spreads into non-sentinel nodes and this causes the node count to increase. Should not be receiving more than three on a regular basis. ![]() ![]() In a small percent of cases the sentinel node may be a small group of two or three nodes. In skilled hands, greater than 98% accurate in identifying sentinel nodes.Done to allow breast conservation therapy in patients with invasive carcinoma
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